Bacteriologist Question:
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Tell me what Are The Conditions When Gram Positive Bacteria Can Appear Gram Negative?

Answer:

☛ When over-decolourized by either prolonged exposure to decolourizer or using acetone alone.
☛ When cell wall gets damaged by exposure to lysozyme or cell wall acting antibiotics such as Penicillin.
☛ Old cultures, where cell wall is weakened or action of autolytic enzymes
☛ Those bacteria that are phagocytosed. where cell wall is acted upon by lysosomal contents

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