Electron Microscopist Interview Preparation Guide Download PDF
Electron Microscopist related Frequently Asked Questions in various Electron Microscopist job Interviews by interviewer. The set of questions here ensures that you offer a perfect answer posed to you. So get preparation for your new job hunting
50 Electron Microscopist Questions and Answers:
1 :: The substage condenser:
A) changes the wavelength of the light reaching the specimen
B) focuses light on the specimen
C) decreases the amount of light reaching the specimen
B) focuses light on the specimen
3 :: Living, unstained cells and organisms can be observed best using:
A) fluorescent microscopy
B) TEM
C) phase contrast microscopy
C) phase contrast microscopy
4 :: Scanning electron microscopy (SEM) is best used to study
A) small internal cell structures.
B) surface morphology.
C) all of the above
B) surface morphology.
5 :: A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a:
A) transmission electron microscope
B) scanning electron microscope
C) phase-contrast microscope
A) transmission electron microscope
7 :: Fixation is the process by which the structures of the cells are preserved and fixed in position. An advantage of chemical fixation over heat fixation is that it:
A) magnifies the specimen
B) does not destroy internal structures
B) does not destroy internal structures
9 :: Which of the following objectives would give you the best resolution of small objects?
A) 10x air, N.A. 0.25
B) 40x air, N.A. 0.65
C) 64x oil, N.A. 1.4
D) 100x oil, N.A. 1.25
C) 64x oil, N.A. 1.4
10 :: Transmission electron microscopy is best for high magnification viewing of:
A) internal structure of fixed cells.
B) internal structure of live, motile cells.
C) surface structure of fixed cells.
D) surface membranes of live, motile cells
A) internal structure of fixed cells.
11 :: Which of the following statements is most correct about the differential Gram stain?
A) Crystal violet differentially stains Gram positive cells.
B) Gram's iodine differentially stains Gram positive cells.
C) Acetone differentially destains Gram negative cells.
D) Saffron red differentially stains Gram negative cells
C) Acetone differentially destains Gram negative cells.
13 :: Which of the following statements about Transmission Electron Microscopy is not true.
A) The specimen must be stained with osmium or other heavy metal.
B) The specimens are placed in a high vacuum for viewing.
C) The specimens must be sliced very thin, 20-100 nm in thickness.
D) The beam is focused by electromagnetic lenses
A) The specimen must be stained with osmium or other heavy metal.
14 :: Phase Contrast microscopy:
A) Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.
C) Uses special lenses to distinguish between solid and liquid phases of the cell.
D) Uses special lenses to change the color of light passing through them
B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.
16 :: "Parfocal" refers to microscopes with multiple objectives where:
A) objectives are used in pairs for stereoscopic effects.
B) each objective has the same working distance above the specimen.
C) each objective is positioned to be in focus at the same stage height.
D) sequential objectives increase power by a factor of two.
C) each objective is positioned to be in focus at the same stage height.
17 :: Differential Interference Contrast microscopy
A) compares two identical specimens on the same microscope.
B) illuminates the specimen with light of two different colors.
C) illuminates the specimen with light of two different phases.
D) illuminates the specimen with both reflected and transmitted light
C) illuminates the specimen with light of two different phases.