Biotechnology Interview Preparation Guide
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Biotechnology Interview Questions and Answers will guide us now that Biotechnology is technology based on biology, agriculture, food science, and medicine. Modern use of the term refers to genetic engineering as well as cell- and tissue culture technologies. So learn more about Biotechnology with the help of this Interview Questions with Answers guide

160 Biotechnology Questions and Answers:

1 :: Where do most allergic reactions occur?

Most of them occur on mucous membrane. Allergens enter the body by the process of inhalation or ingestion.
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2 :: Who are atopic individuals?

Atopic individuals are those who are having abnormal high levels of circulating IgE and more than normal number of oesinophils.
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3 :: What is atopy?

The tendency to manifest localized anaphylactic reactions is called atopy.
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4 :: Name some common allergens associated with type-I hypersensitivity?

Penicillin, sulfonamide, eggs, milk, dust mites, animal air, vaccines etc.
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5 :: What are allergens?

Allergens are non-parasitic antigens. They are capable of stimulating hypersensitive reactions in allergy conditions in an individual.
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6 :: Which type of immunoglobulin level will increase when an individual is exposed to a parasite?

Serum IgE levels will increase and remain until the parasite is washed out from the body.
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7 :: Do you know how the dose for children is being estimated based on preclinical data?

There are a number of ways of estimating children's doses from preclinical (adult) data - often depends on the therapeutic index of the drug in question (the wider the therapeutic window the less accurate the child's dose needs to be). Sometimes straight weight-basis i.e. 7kg child gets 1/10 dose of 70kg adult.

More accurate (so they say) is a dose based on body surface area (child's surface area is greater in proportion to its body weight than an adult is). There are normograms to calculate surface area from weight and height of child.

All of these may be wrong if clearance of drug in child is significantly different from adult e.g. different metabolism or different route of clearance.
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8 :: What is the definition of “Biomedical”? What topics cover the Study of Biomedical Sciences?

The term "biomedical" covers a vast range of subjects - everything that relates biology to medicine. This can range from the obvious like Anatomy, Biochemistry, Physiology, Microbiology, Pharmacology, Genetics to the less obvious like Botany (most drugs were originally derived from plants and, thus, these is a big science called Phytopharmacology).
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9 :: Is Phenoxyethanol harmful?

Phenoxyethanol is harmful and can be absorbed through the skin - official sites for toxicity data, however, show little toxicity in man and some toxicity (irritation) with high doses in animals. Phenoxyethanol is in cosmetics as a bactericide (kills bacteria).
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10 :: Why is buprenorphine less addictive than other opioids (like fentanyl) - is it explainable by its strength of binding to the common receptor, or?

Buprenorphine is what is referred to as a partial agonist - i.e. it binds to the receptor but even at its maximum cannot give as much of an effect as a full agonist (such as morphine) - it is, thus, also a partial antagonist (partially inhibits the actions of full agonists).

As addiction is likely to be linked to strength of the effect of the drug, buprenorphine has less effect and, therefore, less addiction.
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