Analytical Chemistry Question:
Download Questions PDF

What is use of acetonitrile compare to methanol in RP-HPLC Method development?


Answer #1
ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.

Answer #2
Acetonitrile has a higher elution strength than methanol for reversed-phase chromatography, therefore shorter analyte retention can be expected for equal proportions of organic to water

Download Analytical Chemistry Interview Questions And Answers PDF

Previous QuestionNext Question
What is difference between silica used in TLC and HPLC column?How to know HPLC column performance?