Analytical Chemistry Question:
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What is use of acetonitrile compare to methanol in RP-HPLC Method development?

Answers:

Answer #1
ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.

Answer #2
Acetonitrile has a higher elution strength than methanol for reversed-phase chromatography, therefore shorter analyte retention can be expected for equal proportions of organic to water

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