Genetics Question: Download Questions PDF
What is the method used to identify your transformed cells from the plasmid vector?
Answer:
I) If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
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